| 摘要 |
FIELD: biotechnology.SUBSTANCE: group of inventions relates to production of nisin. Disclosed is method and process line for microbiological production of nisin. Method involves culturing of nisin producer in fermenter using nisin producer strain Lactococcus (Streptococcus) lactis, followed by extraction of nisin. Components of nutrient medium represented by brine after drying of dairy milk whey, milk whey, rinsings obtained after process equipment washing at dairy factories, as well as megaterin. Culturing is carried out using automatic pH-stating and thermal regulation, nisin extraction is carried out by acidification with subsequent nisin extraction by centrifugation. Process line contains fermenter with spherical bottom and cover. Fermenter is equipped with bubbler, jacket, mixer and automatic pH-stating and temperature controlling system. Inputs of fermenter are aligned with nutrient medium and seed material feed systems, and output by culture fluid is interconnected through extraction, centrifugation, ultrafiltration and nano filtration units with nisin preparation drying unit.EFFECT: simultaneously with production of nisin inventions provide recycling of previously not used milk production wastes.17 cl, 1 dwg |
