发明名称 |
ON-COLUMN REFOLDING AND PURIFYING OF LIPOPROTEINS |
摘要 |
Provided are methods of refolding and purifying lipoproteins on a chromatography column. In particular methods of refolding ApoL1 polypeptides using a hydrophobic interaction column (HIC). The present invention provides methods for the purification of an active form of a human lipoprotein, such as ApoL1. More particularly, the present invention relates to a method for renaturing an inclusion body of proteins expressed in a large quantity in E. coli into an active form using a hydrophobic interaction column and removal of impurities, e.g., endotoxins. |
申请公布号 |
EP2935323(A4) |
申请公布日期 |
2016.05.25 |
申请号 |
EP20130863926 |
申请日期 |
2013.12.19 |
申请人 |
MERCK SHARP & DOHME CORP. |
发明人 |
CHMIELOWSKI, REBECCA, A.;CUTLER, COLLETTE;LI, HONG;LINDEN, THOMAS, O. |
分类号 |
C07K14/775;C07K1/20 |
主分类号 |
C07K14/775 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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