发明名称 |
Method of producing retinal pigment epithelial cell |
摘要 |
Provided are a method of producing retinal pigment epithelial cells, with which the differentiation-induction efficiency of retinal pigment epithelial cells from pluripotent stem cells can be improved and highly pure retinal pigment epithelial cells obtained in a short time through a simple operation; a culture method for retinal pigment epithelial cells with which the cells can be stably grown and cultured; a toxicity/drug efficacy evaluation method employing retinal pigment epithelial cells useful in transplant therapy; and a retinal disease therapeutic drug. The present invention pertains to a method of producing retinal pigment epithelial cells, including a step for adherent culture of human pluripotent stem cells using a culture substrate coated with laminin E8 fragments; a culture method for retinal pigment epithelial cells, including a step for adherent culture of retinal pigment epithelial cells using a culture substrate coated with laminin E8 fragments; a toxicity/drug efficacy evaluation method employing retinal pigment epithelial cells obtained through production or culture by the aforedescribed methods; and a retinal disease therapeutic drug containing the retinal pigment epithelial cells. |
申请公布号 |
AU2014332856(A1) |
申请公布日期 |
2016.05.19 |
申请号 |
AU20140332856 |
申请日期 |
2014.10.09 |
申请人 |
RIKEN;HEALIOS K.K.;OSAKA UNIVERSITY |
发明人 |
SAWADA, MASANORI;TAKAHASHI, MASAYO;SEKIGUCHI, KIYOTOSHI |
分类号 |
C12N5/10;A61K35/12;A61K35/44;A61P27/02;C12N5/074;C12N5/0775;C12N15/09 |
主分类号 |
C12N5/10 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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