| 摘要 |
The invention relates to a use of one or more nanoparticles (9), each of which is conjugated with at least one oligonucleotide (4), in order to multiply nucleic acids (1). One or more of the oligonucleotides (4) has at least one primer sequence (5) and an additional segment which extends from the end of the primer sequence proximal to the nanoparticle in the direction of the nanoparticle, and the additional segment has at least one abasic modification (7). The invention additionally relates to a method for multiplying nucleic acids (1) in a sample, having a multiplication step for multiplying the nucleic acids and a test step for determining the concentration of the products of the multiplication reaction. The test step begins after the multiplication step ends, and in the test step, either at least one part of the sample is supplied with substances or no substances are supplied. The invention also relates to a method for multiplying nucleic acids (1), wherein nanoparticles (8) transfer heat into their surroundings in a reaction volume (2) upon being excited. The invention finally relates to a method for multiplying a nucleic acid using a polymerase chain reaction, in which a cycle consisting of a denaturing, annealing, and elongating step is repeatedly carried out. |
