DNA-BINDING DOMAIN, NON-FUCOSYLATED AND PARTIALLY FUCOSYLATED PROTEINS, AND METHODS THEREOF

摘要

The present disclosure relates to a method of obtaining a cell where fucosylation pathways are modified, leading to production of partially fucosylated and non-fucosylated protein products, specifically antibodies from the cell. The present disclosure employs the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) technology. The method of the present disclosure targets the Fut8 gene and GMD gene in a cell. Such products are used in developing 10 therapeutics and biomarkers, and in diagnosis and prognosis of diseases.