Method for separating homoduplexed and heteroduplexed nucleic acids

摘要

The invention includes methods and apparatus for separating heteroduplexed nucleic acids from homoduplexed nucleic acids having similar sequences and being at a much higher concentration. The heteroduplexed nucleic acids may be separated through the application of a time varying driving field and a time-varying mobility field to a sample of heteroduplexed and homoduplexed nucleic acids in a separation medium. Once the heteroduplexed nucleic acids are isolated and recovered, it is straightforward to analyze the sequences of the heteroduplexed nucleic acids, e.g., using sequencing or hybrid assays.

主权项

1. A method for separating a mixture of homoduplexed and heteroduplexed nucleic acids, comprising;
loading a sample comprising a mixture of homoduplexed and heteroduplexed nucleic acids on a separation medium; applying a time-varying driving field to the separation medium; and applying a time-varying mobility varying field to the separation medium, thereby causing the homoduplexed nucleic acids to be separated from the heteroduplexed nucleic acids.