IMMUNOSTAINING METHOD, AND IMMUNOSTAINING REAGENT KIT FOR USE IN SAID METHOD

摘要

An azide group (-N3) is introduced into one of an antibody which can be immobilized onto an antigen directly by an antigen-antibody reaction or another antibody which can be immobilized onto the antigen indirectly through the aforementioned antibody and a phosphor-integrated nanoparticle and a carbon-carbon triple bond part (C≡C) is introduced into the other, the antibody is immobilized onto the antigen, a bond through a triazole ring is formed between a molecule of the antibody and a molecule of the phosphor-integrated nanoparticle by a Huisgen cycloaddition reaction between the azide group and the carbon-carbon triple bond part, and the antigen is fluorescently labeled with the phosphor-integrated nanoparticle through the aforementioned formation. In this manner, it becomes possible to prevent the occurrence of non-specific adsorption of a fluorescent dye nanoparticle which may cause the generation of signal noise, and it also becomes possible to prevent the drop out of the bonding between the antibody bonded to the antigen to be detected and the phosphor-integrated nanoparticle and therefore prevent the reduction in signals due to the aforementioned drop out.