发明名称 |
MONOCINS AND METHODS OF USE |
摘要 |
The disclosure relates to the identification, cloning, and expression of a genetic locus within a Listeria monocytogenes genome that encodes a phage tail-like bacteriocin (PTLB), termed a monocin. Also provided are non-natural monocins, which have been engineered to have altered bactericidal specificity. Nucleic acid molecules encoding natural or non-natural monocins, vector constructs containing such nucleic acids operably linked to a heterologous promoter, producer cells containing such vectors, the encoded monocins, as well as methods of making and using such monocins are described. |
申请公布号 |
US2016130309(A1) |
申请公布日期 |
2016.05.12 |
申请号 |
US201514934762 |
申请日期 |
2015.11.06 |
申请人 |
AvidBiotics Corp. |
发明人 |
CHAKRABORTY Urmi;LEE Grace;SCHOLL Dean |
分类号 |
C07K14/195;A01N37/46 |
主分类号 |
C07K14/195 |
代理机构 |
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代理人 |
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主权项 |
1. An isolated nucleic acid molecule encoding a monocin, wherein the nucleic acid molecule comprises a first polynucleotide that encodes the structural proteins of a functional monocin except the corresponding natural receptor binding protein (RBP), wherein the nucleic acid molecule further comprises a heterologous second polynucleotide sequence encoding a heterologous RBP, wherein the RBP comprises a baseplate attachment region (BPAR) and a receptor binding domain (RBD), and wherein the monocin has bactericidal specificity as determined by the heterologous RBP. |
地址 |
South San Francisco CA US |