Hydrolase protein

摘要

It is an object of the present invention to provide a novel hydrolase, which is used when dialkyl 2-vinylcyclopropane-1,1-dicarboxylate is hydrolyzed with an enzyme, so as to efficiently obtain (1S,2S)-1-alkoxycarbonyl-2-vinylcyclopropanecarboxylic acid that is useful as an intermediate for synthesizing therapeutic agents for hepatitis C. According to the present invention, there is provided a hydrolase protein, which consists of the amino acid sequence shown in any one of SEQ ID NOS. 2 to 5 and which has activity of catalyzing, at higher selectivity than the protein consisting of the amino acid sequence shown in SEQ ID NO. 1, a reaction of producing (1S,2S)-1-ethoxycarbonyl-2-vinylcyclopropanecarboxylic acid from diethyl 2-vinylcyclopropane-1,1 -dicarboxylate.

主权项

1. A method for producing (1S,2S)-1-alkoxycarbonyl-2-vinylcyclopropanecarboxylic acid, which comprises contacting dialkyl 2-vinylcyclopropane-1,1-dicarboxylate with a hydrolase protein to produce (1S,2S)-1-alkoxycarbonyl-2-vinylcyclopropanecarboxylic acid, wherein the hydrolase protein
is capable of catalyzing the following reaction at higher selectivity than the protein consisting of the amino acid sequence shown in SEQ ID NO: 1: comprises an amino acid sequence shown in any one of SEQ ID NOs: 2 to 5.