| 摘要 |
FIELD: biotechnology.SUBSTANCE: invention relates to biotechnology, particularly to recombinant production of human proteins, and can be used for production of human SLURP-2 protein. Constructed plasmid DNA pET22b(+)/slurp-2 coding human protein SLURP-2 of 5616 p.a. with physical map presented on fig. 1, which consists of NdeI/HindIII fragment of plasmid DNA pET22b(+) length of 5382 p.a. containing lac-promoter E.coli, gene bla β-lactamase, gene fragment lacI E. coli, section of replication initiation origins and artificial DNA sequence coding NdeI/HindIII fragment length 240 p. representing sequence of synthetic protein gene of human SLURP-2 with SEQ ID NO:7, and has unique recognition sites restricting endonucleases, having following coordinates: XhoI - 158, HindIII - 173, EcoRI - 192, BamHI - 198, NdeI - 430, BglII - 534, MluI - 1256, PstI - 4495. Produced plasmid is used to transform competent cells of E.coli BL21 (DE3) to produce bacterial strain E.coli BL21(DE3)/pet22b(+)/slurp-2, producer of human protein SLURP-2.EFFECT: invention enables higher effectiveness of SLURP-2 protein synthesis in Escherichia coli.2 cl, 4 dwg, 6 ex |
