Method of producing lipids using a thioesterase variant

摘要

A method of producing a lipid, comprising steps of
introducing a gene that encodes the following protein (a) or (b) into a host, and thereby obtaining a transformant, andcollecting a lipid from the transformant:(a) A protein comprising an amino acid sequence of the 112nd to 414th amino acids set forth in SEQ ID NO: 1 in which an amino acid corresponding to the 209th amino acid set forth in SEQ ID NO: 1 is substituted from tryptophan to threonine, glutamic acid or alanine, and having thioesterase activity, and(b) A protein comprising an amino acid sequence of the protein (a) in which one to several amino acids other than the amino acid corresponding to the 209th amino acid set forth in SEQ ID NO: 1 are deleted, substituted, inserted or added, and having thioesterase activity.

主权项

1. A method of producing a lipid, comprising steps of introducing a gene that encodes the following protein (a) or (b) into a host, and thereby obtaining a transformant,
culturing or growing the transformant to obtain a culture or grown product, and collecting a lipid from the culture media, grown product or transformant, wherein the proteins (a) and (b) are: (a) A protein comprising an amino acid sequence of the 112nd to 414th amino acids set forth in SEQ ID NO: 1 in which an amino acid corresponding to the 209th amino acid set forth in SEQ ID NO: 1 is substituted from tryptophan to threonine, glutamic acid or alanine, and having thioesterase activity, and (b) A protein comprising an amino acid sequence of the protein (a) in which one to ten amino acids other than the amino acid corresponding to the 209th amino acid set forth in SEQ ID NO: 1 are deleted, substituted, inserted or added, and having thioesterase activity.