Multiple Phase Systems and Kits

摘要

Multi-phase systems and kits using these multiphase systems are described. The Multi-phase system described herein comprises multiple phase-separated phases each comprises a phase component and the phases, taken together, represent a density gradient. The kit comprising the multiphase system as described herein may be used to separate biological analytes such as cells. Non-limiting examples of the biological analytes include normal erythrocyte with hemoglobin Hb AA, Hb CC, and Hb AS, sickle cell erythrocyte with hemoglobin Hb SS and Hb SC, reticulocyte, iron deficiency anemia red blood cell, β-thalessemia trait red blood cell, and normal red blood cell.

主权项

1. An aqueous multi-phase system for diagnosis of iron deficiency anemia, comprising two or more phase-separated phases comprising:
a first aqueous phase comprising a first phase component and having a first density between about 1.025 g/cm3 and about 1.085 g/cm3; and a second aqueous phase comprising a second phase component and having a second density between about 1.075 g/cm3 and about 1.095 g/cm3; wherein the first density is lower than the second density; and each of the first and second phase components comprises at least one polymer.