| 主权项 |
1. A chimeric influenza hemagglutinin segment having an ectodomain, a 5′-non-coding region, a 3′-non-coding region, a signal peptide, a transmembrane domain and a cytoplasmic domain wherein (1) the ectodomain is from a first influenza strain and one or more of the 5′-non-coding region, the 3′-non-coding region, the signal peptide, the transmembrane domain and the cytoplasmic domain are from a second influenza strain which is not A/Puerto Rico/8/34, A/WSN/33 or B/Lee/40, wherein the first and the second influenza strains are both influenza A or influenza B strains, (2) the ectodomain is from a first influenza A strain which is not a H1 or H5 influenza strain, and one or more of the 5′-non-coding region, the 3′-non-coding region, the signal peptide, the transmembrane domain and the cytoplasmic domain are from a second influenza strain, wherein the first and the second influenza strains are both influenza A or influenza B strains, (3) the ectodomain is from a first influenza B strain, and one or more of the 5′-non-coding region, the 3′-non-coding region, the signal peptide, the transmembrane domain and the cytoplasmic domain are from a second influenza strain which is an influenza B strain or an influenza A strain which is not a H1 strain or a H3 strain, or (4) the ectodomain is from a first influenza strain and one or more of the 5′-non-coding region, the 3′-non-coding region, the signal peptide, the transmembrane domain and the cytoplasmic domain are from a second influenza strain, wherein the segment comprises one or more of:
a) guanine in the position corresponding to nucleotide 24 of SEQ ID NO: 15, and/or b) adenine in the position corresponding to nucleotide 38 of SEQ ID NO: 15; and/or c) thymine in the position corresponding to nucleotide 40 of SEQ ID NO: 15; and/or d) adenine in the position corresponding to nucleotide 44 of SEQ ID NO: 15; and/or e) thymine in the position corresponding to nucleotide 53 of SEQ ID NO: 15; and/or f) adenine in the position corresponding to nucleotide 63 of SEQ ID NO: 15; and/or g) thymine in the position corresponding to nucleotide 66 of SEQ ID NO: 15; and/or h) adenine in the position corresponding to nucleotide 69 of SEQ ID NO: 15; and/or i) adenine in the position corresponding to nucleotide 75 of SEQ ID NO: 15; and/or j) thymine in the position corresponding to nucleotide 78 of SEQ ID NO: 15; and/or k) adenine in the position corresponding to nucleotide 1637 of SEQ ID NO: 15, and/or l) cytosine in the position corresponding to nucleotide 1649 of SEQ ID NO: 15, and/or m) thymine in the position corresponding to nucleotide 1655 of SEQ ID NO: 15, and/or n) cytosine in the position corresponding to nucleotide 1682 of SEQ ID NO: 15, and/or o) cytosine in the position corresponding to nucleotide 1697 of SEQ ID NO: 15, and/or p) guanine in the position corresponding to nucleotide 1703 of SEQ ID NO: 15, and/or q) thymine in the position corresponding to nucleotide 1715 of SEQ ID NO: 15, and/or r) adenine in the position corresponding to nucleotide 1729 of SEQ ID NO: 15, and/or s) cytosine in the position corresponding to nucleotide 1733 of SEQ ID NO: 15, and/or t) cytosine in the position corresponding to nucleotide 1734 of SEQ ID NO: 15, and/or u) adenine in the position corresponding to nucleotide 1746 of SEQ ID NO: 15, and/or v) adenine in the position corresponding to nucleotide 1751 of SEQ ID NO: 15;when aligned to SEQ ID NO: 15 using a pairwise alignment algorithm. |
