| 摘要 |
Novel CARD-9, CARD-10, or CARD-11 polypeptides, proteins, and nucleic acid molecules are disclosed. In addition to isolated CARD-9, CARD-10, or CARD-11 proteins, the invention further provides CARD-9, CARD-10, or CARD-11, fusion proteins, antigenic peptides and anti-CARD-9, CARD-10, or CARD-11 antibodies. The invention also provides CARD-9, CARD-10, or CARD-11 nucleic acid molecules, recombinant expression vectors containing a nucleic acid molecule of the invention, host cells into which the expression vectors have been introduced and non-human transgenic animals in which a CARD-9, CARD-10, or CARD-11 gene has been introduced or disrupted. Diagnostic, screening and therapeutic methods utilizing compositions of the invention are also provided. |
| 主权项 |
1. A method for detecting in a sample the presence of a polypeptide selected from the group consisting of:
a) a polypeptide comprising a fragment of the amino acid sequence of SEQ ID NO:11, wherein the fragment comprises the CARD domain of residues 6-112 of SEQ ID NO:11, wherein the fragment binds Bcl-10; b) a polypeptide comprising a variant of the amino acid sequence of SEQ ID NO:11, wherein the polypeptide is encoded by a nucleic acid molecule which hybridizes to a nucleic acid molecule consisting of the entire complement of SEQ ID NO:12 under conditions of incubation at 45° C. in 6.0×SSC followed by washing in 0.2×SSC/0.1% SDS at 65° C., wherein the variant binds Bcl-10 and comprises a CARD domain; c) a polypeptide which is encoded by a nucleic acid molecule comprising a nucleotide sequence which is at least 95% identical to SEQ ID NO:10 or 12, wherein the polypeptide binds Bcl-10 and comprises a CARD domain; and d) a polypeptide comprising an amino acid sequence that is at least 95% identical to SEQ ID NO:11, wherein the polypeptide binds Bcl-10 and comprises a CARD domain, comprising: i) contacting the sample with a compound which selectively binds to the polypeptide; and ii) determining whether the compound binds to the polypeptide in the sample, wherein the compound is an isolated antibody which selectively binds to the polypeptide. |
