发明名称 |
A MICROFLUIDIC DEVICE WITH A DIFFUSION BARRIER |
摘要 |
The invention provides a microfluidic device for macromolecule amplification by sequential addition of liquid reagents. The device of the invention comprises a chip forming a plurality of reaction chambers each extending between an inlet and an outlet, each inlet being in fluid communication with a common junction via micro channels. To enable amplification of DNA, e.g. by MDA, the device comprises a diffusion barrier at each inlet configured to increase the pressure threshold for a reagent to cross the resistor. The invention further provides a method of mixing liquid reagents by use of the device where single DNA molecules are allowed to cross the diffusion barrier individually. |
申请公布号 |
EP3013476(A1) |
申请公布日期 |
2016.05.04 |
申请号 |
EP20140734800 |
申请日期 |
2014.06.30 |
申请人 |
DANMARKS TEKNISKE UNIVERSITET |
发明人 |
ERIKSEN, JOHAN;MARIE, RUDOLPHE;KRISTENSEN, ANDERS |
分类号 |
B01L3/00 |
主分类号 |
B01L3/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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