发明名称 |
METHOD AND DEVICE FOR COLLECTION AND AMPLIFICATION OF CIRCULATING NUCLEIC ACIDS. |
摘要 |
Provided herein are methods for the collection and amplification of circulating nucleic acids from a non-celluar fraction of a biological sample. Circulating nucleic acids are extracted from the non-cellular fraction and are circularized to generate single-stranded nucleic acid circles, which are then subsequently amplified by rolling circular amplification using random primers to produce an amplified library. Devices for the collection of a non-cellular fraction from a bilogical sample are also provided. The device includes a filtration membrane and a dry solid matrix, which is in direct contact with the filtration membrane. |
申请公布号 |
MX2016001159(A) |
申请公布日期 |
2016.04.29 |
申请号 |
MX20160001159 |
申请日期 |
2014.07.24 |
申请人 |
GENERAL ELECTRIC COMPANY |
发明人 |
RYAN CHARLES HELLER;JOHN RICHARD NELSON;ERIK LEEMING KVAM;GREGORY ANDREW GROSSMANN;ERIN JEAN FINEHOUT;CHRISTOPHER MICHAEL PULEO;WILLIAM PATRICK WATERS |
分类号 |
C12Q1/68;B01D11/04;C12N15/10 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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