Methods of Reducing Methylglyoxal (MGO) Modification of Recombinant Proteins in Cell Culture

摘要

Methods are provided for controlling the levels of acidic species when a protein of interest is expressed by host cells. One particular example of an acidic species is methylglyoxal (MGO). Reduction of acidic species such as MGO may be achieved by regulating the glucose consumption of the cell culture or by modulating the cysteine/glutathione metabolic pathways.

主权项

1. A method for reducing methylglyoxal (MGO) modification in adalimumab produced by cell culture in chemically defined media, comprising reducing the rate of glucose consumption during cell culture.