Method for Making Mature Insulin Polypeptides

摘要

This invention relates to an improved method for making mature human insulin or an analogue thereof by cultivating fungi cell comprising a DNA sequence encoding a precursor for human insulin or an analogue thereof, which precursor comprises a small connecting peptide.

主权项

1. An insulin precursor comprising the sequence Z-B-X-Y-A, wherein
Z is an optional extension sequence, B is the B-chain of human insulin or an analogue thereof, X is a sequence selected from the group consisting of X1M, EA, AE, AD, DA, and AP, where X1 is a sequence comprising from 1 to 3 amino acid residues, Y is K or R, and A is the A-chain of human insulin or an analogue thereof.