MICROFLUIDIC DEVICE AND ARRAY DISK

摘要

The invention relates to microfluidic devices and array disks for “one-pot” isolated chemical reactions. The array disks comprise a plurality of sectors in which each sector comprises one microfluidic device. The microfluidic devices comprise a fluid delivery channel and an array of wells wherein the fluid delivery channel delivery fluid into the wells in a serpentine arrangement. In some embodiments, the fluid delivery channel is directly above the array of wells. In other embodiments, the fluid delivery channel is offset from the array of wells so that side channels branching from the fluid delivery channel delivers fluid into the wells. The well of the microfluidic device comprises a gas-permeable membrane that forms the floor, well, or at least a portion of the floor or wall of the well. In preferred embodiments, the well is cylindrical.

主权项

1. A microfluidic device comprising:
a fluid delivery channel; an array of wells arranged to follow the serpentine flow of a fluid delivery channel, wherein each well of the array of wells comprises:
a hydrophilic coating;a gas-permeable membrane;a top opening; anda bottom opening, wherein the bottom opening is sealed by the gas-permeable membrane to form the floor of each well; and a reservoir, wherein the top opening of each well meets fluid delivery channel; the reservoir is below gas-permeable membrane; and the fluid delivery channel directs a fluid into each well in a serpentine order whereby an entire column of wells is filled before wells in the next column are filled.