Simultaneous saccharification and fermentation(SSF) of lignocellulosic biomass for single cell oil production by oleaginous microorganisms

摘要

Methods for producing lipids from lignocellulosic biomass are provided. Sugars produced by pretreatment of lignocellulosic biomass are utilized by heterotrophic oleaginous fungi or yeast to increase their biomass and to produce lipids without prior detoxification of the pretreated biomass. After the fungi/yeast are cultured with the sugars, solid residues from the pretreated biomass are combined with the fungi/yeast under conditions which allow simultaneous 1) enzymatic degradation of cellulose and/or hemicellulose to produce sugars and 2) fermentation of the sugars for further increases in biomass and lipid production.

主权项

1. A method of producing lipids from lignocellulosic biomass, comprising the steps of:
i) pretreating said lignocellulosic biomass to form a lignocellulosic feedstock; ii) separating said lignocellulosic feedstock into a solids fraction and a liquid fraction; iii) culturing at least one species of oleaginous yeast or fungus in culture medium comprising said liquid fraction under conditions that allow said oleaginous yeast or fungus to produce and accumulate lipids, iv) culturing Cryptococcus curvatus in culture medium comprising said solids fraction and one or more cellulase and/or hemicellulase enzymes under conditions which allow said cellulase and/or hemicellulase enzymes to hydrolyze cellulose and/or hemicellulose in said solids fraction, and which allow said Cryptococcus curvatus to employ fermentation to produce and accumulate lipids in an amount of 124 to 151 mg/g, and v) extracting said lipids produced in steps iii) and iv) from said lignocellulose biomass.