AUTOMATED ISOLATION AND CHEMICAL REACTION(S) OF NUCLEIC ACIDS

摘要

The present teachings relate to methods, kits and devices for performing automated sequential nucleic acid isolation and conversion/purification in a single closed system. In various embodiments, the present teaching enable a user to (i) load a device with test samples, reagents and consumables; (ii) select or program the device for the desired nucleic acid isolation and subsequent chemical treatment and/or conversion reaction(s) without further user intervention; and recovering the isolated and treated and/or converted nucleic acid at the conclusion of the program once the device is activated.

主权项

1. A method for performing sequential nucleic acid isolation and conversion/purification reaction(s) in a single closed system, wherein an isolated nucleic acid is prepared for modification detection, comprising:
a. reacting the nucleic acid-containing sample with an extraction reagent, wherein an isolated nucleic acid is obtained from the sample, and b. reacting said isolated nucleic acid with a conversion reagent .wherein the said nucleic acid having the modification is prepared for further detection.