| 摘要 |
The present invention provides a primer for nucleic acid random fragmentation and a nucleic acid random fragmentation method. The primer consists of a plurality of upstream random primers and downstream random primers. The sequence composition of the upstream random primers is 5'-X-Y-3', and the sequence composition of the downstream random primers is 5'-P-Y'-X'-close-3', wherein Y and Y' are random sequences, X is all or part of sequences of a sequencing platform 5' end adaptor, X' is all or part of sequences of a sequencing platform 3' end adaptor, P is phosphorylation modification, and close is close modification. The primer of the present invention adopts double random anchoring of both the upstream random primers and the downstream random primers, and a DNA sample can be randomly broken. |
