| 发明名称 |
METHODS AND COMPOSITIONS FOR GENERATING OR MAINTAINING PLURIPOTENT CELLS |
| 摘要 |
Methods and compositions are provided for generating or maintaining human iPS cells in culture. Methods include the use of a low osmolality medium to make human iPS cells, or use of a low osmolality medium to maintain human iPS cells. Methods for making targeted genetic modification to human iPS cells cultured in low osmolality medium are also included. Compositions include human iPS cells cultured and maintained using the low osmolality medium defined herein. |
| 申请公布号 |
US2016108369(A1) |
申请公布日期 |
2016.04.21 |
| 申请号 |
US201514884293 |
申请日期 |
2015.10.15 |
| 申请人 |
REGENERON PHARMACEUTICALS, INC. |
发明人 |
Kuno Junko;Auerbach Wojtek;Valenzuela David M. |
| 分类号 |
C12N5/074 |
主分类号 |
C12N5/074 |
| 代理机构 |
|
代理人 |
|
| 主权项 |
1. An in vitro culture comprising:
(a) a population of hiPSCs; and (b) a low osmolality medium comprising a base medium and supplements, wherein the low osmolality medium comprises:
(i) a leukemia inhibitory factor (LIF) polypeptide;(ii) a glycogen synthase kinase 3 (GSK3) inhibitor; and(iii) a MEK inhibitor;wherein the base medium has an osmolality of about 180 mOsm/kg to about 250 mOsm/kg. |
| 地址 |
Tarrytown NY US |
