EXPRESSION OF RECOMBINANT TETRACYCLINE EFFLUX PUMPS FOR THE PRODUCTION OF LYSINE OR LYSINE-DERIVED PRODUCTS, AND METHODS AND APPLICATIONS THEREOF

摘要

The present invention provides a mutant polypeptide comprising the amino acid sequence of Escherichia coli tetracycline efflux pump A (TetA), and a polynucleotide encoding the polypeptide. The present invention provides a first expression plasmid vector comprising one or more first polynucleotides encoding a tetracycline efflux pump, one or more second polynucleotides independently selected from the group consisting of a third polynucleotides encoding a lysine decarboxylase polypeptide and a fourth polynucleotides encoding a lysine biosynthesis polypeptide, and a transformant comprising the expression plasmid vector. The present invention provides a host cell comprising one or more first, third or fourth polynucleotides as described herein integrated into a chromosome of the host cell. The present invention also provides a method for producing a lysine of a cadaverine using the transformant and/or the host cell.