MICROFLUIDIC DEVICE AND SYSTEM FOR PRECISELY CONTROLLING AND ANALYZING SHEAR FORCES IN BLAST-INDUCED TRAUMATIC BRAIN INJURIES

摘要

Embodiments of a microfluidic system and method for stimulating a blast shock wave that supports a short and defined laminar flow of a liquid media solution through a microfluidic device such that sheer stress on neural tissue disposed within the microfluidic device is precisely controlled are disclosed. The microfluidic system includes a pneumatic device applies a blast shock wave having a quick rise time across a microfluidic channel of the microfluidic device. The microfluidic device includes an inlet reservoir in fluid flow communication with an outlet reservoir through the microfluidic channel. The inlet and outlet reservoirs are secured to a top structure which is attached to a bottom structure that collectively defines the microfluidic channel with a cover slip that is attached to the underside of the bottom structure.

主权项

1. A microfluidic system for precisely controlling shear forces generated by a blast shock wave, the system comprising:
a microfluidic device comprising:
a top structure attached to a bottom structure in which the bottom structure defines a microfluidic channel having a first end and a second end, the microfluidic channel including an inlet at the first end and an outlet at the second end;an inlet reservoir engaged to the top structure and in fluid communication with the inlet of the microfluidic channel, wherein the inlet reservoir includes a baffle defining a restricted opening; andan outlet reservoir engaged to the top structure and in fluid communication with the outlet of the microfluidic channel; a liquid media solution that fills the microfluidic device; and a pneumatic device in fluid communication with the microfluidic device to deliver a pressurized gas that generates a blast shock wave having a quick rise and fall in pressure that causes a short and fast movement of the liquid media solution through the restricted opening of the baffle and supports a laminar flow of the liquid media solution through the microfluidic channel.