METHODS AND COMPOSITIONS FOR ISOLATING SMALL RNA MOLECULES

摘要

The present invention concerns the use of methods and compositions for the isolation of small RNA molecules (100 nucleotides or fewer), such as microRNA and siRNA molecules. Such molecules are routinely lost in commonly used isolation procedures and therefore the present invention allows for a much higher level of enrichment or isolation of these small RNA molecules.

主权项

1. A method for isolating small RNA molecules from cells comprising:
a) lysing the cells with a lysing solution to produce a lysate; b) adding an alcohol solution to the lysate; c) applying the lysate to a solid support; d) eluting small RNA molecules from the solid support; and, e) using or characterizing the small RNA molecules.