TECHNIQUE FOR DETERMINING THE STATE OF A CELL MASS, IMAGE PROCESSING PROGRAM AND IMAGE PROCESSING DEVICE USING SAID TECHNIQUE, AND METHOD FOR PRODUCING A CELL MASS

摘要

Provided is means for determining and outputting, from time lapse images, the state of change of a cell aggregation toward becoming multi-layered. An image processing program (GP) is provided with: a step (S10) for obtaining time lapse images; a step (S20) for extracting a cell aggregation from each of the obtained images; a step (S50) for calculating, for the entire region of the cell aggregation, the spatial distribution in pixel values and temporal change in pixel values of a small region in the extracted cell aggregation; a step (S70) for determining the state of change of the cell aggregation toward becoming multi-layered, on the basis of the calculated spatial distribution and time lapse change of pixel values; and a step (S80) for outputting the results of the determination. The image processing program (GP) is configured so as to determine and output, from time lapse images, the state of change of the cell aggregation toward becoming multi-layered.