Chromosomal insertion of GFP into bacteria for quality control

摘要

An isolated mutated green fluorescent protein (gfp) gene for chromosomal insertion into a bacterium, wherein the gene is capable of being expressed in bacteria and produce sufficient fluorescence under illumination from a UV lamp in a bacterial colony to be seen by the naked eye. A gene cassette for inserting a gene into a chromosome.

主权项

1. An isolated modified bacterium forming a colony having fluorescence visible by the naked eye when the colony is illuminated by ultraviolet light, the modified bacterium comprising a mutated green fluorescent protein (GFP) gene from the jellyfish Aequorea victoria inserted into the chromosome of the modified bacterium; wherein the mutated GFP gene comprising a nucleic acid molecule consisting of mutations at nucleotide positions 1737 and 1739 of SEQ ID NO: 4 encoding Ser72Ala, nucleotide position 1981 of SEQ ID NO: 4 encoding Met153Thr and nucleotide position 2011of SEQ ID NO: 4 encoding Val163Ala, or the mutated GFP gene comprising a nucleic acid molecule consisting of mutations which encode amino acid changes Ser72Ala, Met153Thr, Val163 Ala of SEQ ID NO: 3, and wherein the mutated GFP gene is under the control of an un-repressed strong promoter.