Water soluble membrane proteins and methods for the preparation and use thereof

摘要

The present invention is directed to water-soluble membrane proteins, methods for the preparation thereof and methods of use thereof.

主权项

1. A water-soluble variant of a G-protein coupled receptor (GPCR), wherein in said variant, 7-transmembrane α-helical hydrophobic residues Leucine (L), isoleucine (I), valine (V), and phenylalanine (F) in hydrophilic surface α-helical positions b, c, and f but not positions a, d, e, and g of the GPCR have been substituted by glutamine (Q), threonine (T), threonine (T), and tyrosine (Y), respectively, optionally further comprising conservative substitutions at other parts of the variant.