| 摘要 |
The invention discloses a noninvasive cancer detection method, which comprises the following steps: acquiring peripheral blood of a subject and separating blood plasma; extracting blood plasma DNA and processing by using bisulfate; amplifying the blood plasma DNA processed by bisulfite through polymerase to prepare a sequencing library; performing high flux sequencing on the prepared sequencing library; contrasting a sequence obtained through high flux sequencing with human genome; making statistics for a methylation level of a genome specific area in the sequence obtained through the high flux sequencing, contrasting with the methylation level in genome unit length determined by healthy population, and determining whether the subject suffers from cancer or not. The invention also discloses a kit for the above method. |
