| 摘要 |
The invention discloses a nanometer PCR detection kit for rapidly identifying and diagnosing virulent virus and attenuated virus of porcine pseudorabies virus, and applications thereof. The kit comprises a 2*Nano PCR buffer, Taq DNA polymerase, a primer mixture, DNA polymerase, and nucleic acid-free ddH2O. In order to achieve a good detection effect, the kit further can contain a template mixture and a stabilizer. With the kit of the present invention, disadvantages of long time consuming, tedious property and low sensitivity of the existing kit are solved, sensitivity is improved, and early stage and rapid virus detection is achieved. The kit of the present invention has characteristics of strong specificity, high sensitivity and the like, is suitable for early stage and rapid diagnosis, and can be provided for identifying gene deletion strains and wild strain infection. In addition, the kit can be widely used in grassroots veterinary detection institutions, and can provide important significance for PRV detection, diagnosis and identifying diagnosis in large-scale pig farms. |
