MEANS AND METHODS FOR PRODUCING HIGH AFFINITY ANTIBODIES

摘要

The invention provides means and methods for producing high-affinity antibodies against an antigen of interest, usually stable B-cell cultures.

主权项

1. An isolated or recombinant antibody-producing B-cell produced by the method comprising:
a) selecting a B-cell able to produce and antibody specific for an antigen of interest or selecting a B-cell able to differentiate into a B-cell that is able to produce antibody specific for said antigen of interest; b) inducing, enhancing and/or maintaining expression of BCL6 in said B-cell; c) inducing, enhancing and/or maintaining expression of an anti-apoptotic nucleic acid in said B-cell; d) allowing expansion of said B-cells into a first population of B-cells; e) performing antigen staining and BCR staining of B-cells from said first population; f) selecting a B-cell that has a high antigen affinity; g) allowing expansion of said B-cell into a second population of B-cells; h) measuring the antigen association rate and dissociation rate of antibodies produced by at least one B-cell from said second population; and i) selecting an antibody with an affinity for said antigen of interest that is higher than the average affinity of said first population of B-cells for said antigen of interest