EXPRESSION OF HETEROLOGOUS MICROBIAL OXIDATIVE ENZYMES TO INCREASE THE AMOUNT OF BIOLOGICALLY LABILE ORGANIC CARBON FROM PRETREATED COAL

摘要

A method for treating a low value carbon source to produce biologically labile carbon, comprising the steps of oxidizing the low value carbon source with at least one oxidizing agent to produce a modified, partially solubilized low value carbon source; and contacting the modified, partially solubilized low value carbon source with a microorganism adapted for growth in the modified, partially solubilized low value carbon source, which expresses at least one oxidative enzyme. A genetically engineered microorganism is also disclosed which provides increased oxidative enzyme activity and an enhanced expression level for an ancillary protein, relative to the corresponding wild-type microorganism.