PROCESS AND SYSTEM FOR OBTAINING BOTULINUM NEUROTOXIN

摘要

Rapid, animal protein free, chromatographic processes and systems for obtaining high potency, high yield botulinum neurotoxin for research, therapeutic and cosmetic use.

主权项

1. A substantially animal product free (APF) process utilizing chromatography for obtaining a botulinum neurotoxin, the process comprising the following steps:
(a) providing a substantially APF fermentation medium; (b) fermenting clostridium botulinum bacteria in the fermentation medium, wherein the clostridium botulinum bacteria produce a botulinum neurotoxin, and; (c) sequentially contacting a plurality of chromatography resins with an aqueous medium containing the botulinum neurotoxin, wherein the plurality of chromatography resins comprise an ion exchange resin, a hydrophobic interaction resin, a gel filtration resin, or combinations thereof; thereby obtaining the botulinum neurotoxin from the substantially APF chromatography process.