发明名称 |
NOVEL MEASUREMENT METHOD USING KINASE, AND COMPOSITION |
摘要 |
Provided is a measurement method for at least one of a forward reaction substrate for a kinase, a phosphorus oxide thereof, and derivative precursors that are converted into the aforementioned substances. The measurement method includes: a step in which at least a kinase, a first nucleotide coenzyme of the kinase, and a second nucleotide coenzyme that has a different nucleoside moiety than the first nucleotide coenzyme are brought into contact with a sample and an enzymatic cycling reaction is carried out; a step in which a signal corresponding to the amount of change between the first nucleotide coenzyme and the transformed product thereof and/or between the second nucleotide coenzyme and the transformed product thereof is detected; and (3) a step in which the amount of a forward reaction substrate for the kinase and/or a phosphorus oxide thereof included in the sample is calculated on the basis of the amount of change in the detected signal. |
申请公布号 |
WO2016047580(A1) |
申请公布日期 |
2016.03.31 |
申请号 |
WO2015JP76639 |
申请日期 |
2015.09.18 |
申请人 |
ASAHI KASEI PHARMA CORPORATION |
发明人 |
UEDA, SHIGERU;SAKASEGAWA, SHINICHI |
分类号 |
C12Q1/48;C12M1/34;C12N9/02;C12N9/04;C12N9/12;C12Q1/26;C12Q1/54;C12Q1/61;C12Q1/68 |
主分类号 |
C12Q1/48 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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