| 摘要 |
A kit for optically detecting proteins, in particular lipoproteins, in a sample, the kit comprising: a chip for performing a separation of the proteins, in particular of the lipoproteins, wherein the chip comprises at least one well for receiving the sample, and a separation channel coupled to the at least one well and being adapted for separating different compounds and a marker dye which contains a polymethine of the general formula (I) wherein Z is a substituted derivative of benzooxazole, benzodiazole, 2,3,3-trimethylindolinine, 2,3,3-trimethyl-4,5-benzo-3H-indolinine, 3- and 4-picoline, lipidine, chinadine and 9-methylacridine derivatives with the general formula IIa or IIb or IIc and wherein X is selected from the group consisting of O, S, Si, N-alkyl and C(alkyl)2, n is 0, 1 , 2 or 3, R1 to R14 are independently selected from the group consisting of hydrogen, alkyl, alkoxy, cycloalkyl, linear or branched alkenyl, cycloalkenyl, aryl, heteroaryl, heterocycle, hydroxy, carboxy, amine, alkyl-substituted amine and cyclic amine and/or two or more fragments in ortho-position to each other, for example R10 and R11 or R4, R5 and R6, together form another cycloalkyl ring or ring system, heterocyclic ring or ring system, heteroaryl ring system or aromatic ring or ring system.; |
| 主权项 |
1. A method of analyzing lipoproteins in a sample, the method comprising:
labeling the lipoproteins in a sample with a marker dye containing a polymethine of the general formula I wherein Z has the general formula IIa and wherein X is C(CH3)2, n is 1, R1 is —C(CH3)3; R2, R3, R4, R6, R7, R8, R9, R10, R11, R12 and/or R13 is hydrogen; R5 is —N(CH2CH3)2; R14 is —(CH2)3—OH, —(CH2)5—COOH, or —CH3; performing on a microfluidic device a separation of the labeled lipoproteins in the sample; and optically detecting the separated labeled lipoproteins. |
