VIRAL VECTORS ENCODING MULTIPLE HIGHLY HOMOLOGOUS NON-VIRAL POLYPEPTIDES AND THE USE OF SAME

摘要

The present invention relates to compositions and methods for creation of vector nucleic acid sequences (e.g., retroviral nucleic acid sequences) that comprise two or more exogenous nucleic acid sequences that encode highly homologous (e.g., identical) polypeptide sequences, yet wherein at least one of the exogenous nucleic acid sequences has been mutated using degenerate codons for purpose of reducing homology between the two or more exogenous nucleic acid sequences while maintaining the encoded polypeptide sequence. Preferred nucleic acid sequences include those encoding multi-chimeric immune receptor (CIR) genes. Specific nucleic acid sequences of such CIR genes are also disclosed.

主权项

1. A retroviral vector comprising:
a first nucleic acid sequence that encodes a first polypeptide sequence and a second nucleic acid sequence that encodes a second polypeptide sequence, wherein: (a) the first polypeptide sequence and the second polypeptide sequence comprise an identical polypeptide sequence of greater than 15 amino acids in length; and (b) the first nucleic acid sequence and the second nucleic acid sequence contain at least one non-identical codon within the codons that encode the identical polypeptide sequence of the first and second nucleic acid sequences.