| 发明名称 | Engineered microorganisms having resistance to ionic liquids | ||
| 摘要 | The present invention provides for a method of genetically modifying microorganisms to enhance resistance to ionic liquids, host cells genetically modified in accordance with the methods, and methods of using the host cells in a reaction comprising biomass that has been pretreated with ionic liquids. | ||
| 申请公布号 | US9290768(B2) | 申请公布日期 | 2016.03.22 |
| 申请号 | US201313935325 | 申请日期 | 2013.07.03 |
| 申请人 | Lawrence Livermore National Security, LLC | 发明人 | Ruegg Thomas Lawrence;Thelen Michael P. |
| 分类号 | C12P1/00;C12P7/10;C12N15/70;C07K14/265;C12N1/22 | 主分类号 | C12P1/00 |
| 代理机构 | Kilpatrick Townsend & Stockton LLP | 代理人 | Kilpatrick Townsend & Stockton LLP |
| 主权项 | 1. A method of increasing the yield from a reaction in which soluble sugars are a source of carbon, the method comprising incubating a microorganism with biomass pretreated with an ionic liquid, wherein the biomass pretreated with an ionic liquid provides a source of soluble sugars as a carbon source, wherein the microorganism comprises a heterologous gene encoding a Major Facilitator Superfamily 1 (MFS 1) polypeptide operably linked to a promoter, wherein said MFS 1 polypeptide has an amino acid sequence with at least 95% sequence identity to the amino acid sequence set forth in SEQ ID NO:2, and wherein said MFS 1 polypeptide facilitates resistance of the microorganism to said ionic liquid as compared to a microorganism without said heterologous gene. | ||
| 地址 | Livermore CA US | ||