Method for manufacturing multilayered cell sheet, multilayered cell sheet having vascular network obtained thereby, and method of use thereof

摘要

A method for producing multilayered cell sheets, including producing a vascular bed which includes an artery-vein loop and in which a capillary vascular network is constructed; layering cell sheets on the vascular bed; and perfusing a culture medium in vitro to construct a vascular network in the cell sheets. The production method enables vascular networks to be constructed in cell sheets and enables thick multilayered cell sheets to foe easily produced by layering the cell sheets. Such thick multilayered cell sheets are useful as in vivo tissue-like products for regenerative medicine for various tissues and for evaluation of drugs and the like.

主权项

1. A multilayered cell sheet product comprising a vascular bed, multiple layers of cell sheets layered on the vascular bed, and a capillary vascular network in the cell sheets,
wherein the vascular bed comprises a biological tissue with a femoral artery-vein loop and circulating culture medium, and wherein the capillary vascular network is generated by layering the cell sheets on the vascular bed and perfusing the culture medium in vitro into the vascular bed to induce the capillary vascular network into the cell sheets.