| 摘要 |
FIELD: biochemistry.SUBSTANCE: invention is a method for rapid determination of the resistance to oxidation of low density lipoproteins of serum by treatment with a buffer containing polyvinylpyrrolidone (PVP), followed by turbidimetric registration mixture, characterized in that the treatment of serum or human plasma are 15% solution of PVP-12600 to 0.01M Tris-HCl-buffer, pH 7.4, containing 0.15 M NaCl, at a volume ratio of serum: PVP (1:6) 10 and incubated for 20 minutes at room temperature, the absorbance is measured spectrophotometrically at 450 nm in the experimental and control samples is calculated and the difference between them in the absence of difference ascertain normal resistance to oxidation of LDL in human blood serum.EFFECT: embodiment of the invention makes it possible to expand the arsenal of these methods, as well as reduce the complexity of the analysis.1 cl, 4 ex, 4 tbl |
