发明名称 |
COENZYME-LINKED GLUCOSE DEHYDROGENASE AND POLYNUCLEOTIDE ENCODING THE SAME |
摘要 |
The present invention provides members that produce on a large scale a coenzyme-linked glucose dehydrogenase which has excellent substrate-recognizing ability toward glucose while providing low action on maltose. The present invention relates to a polynucleotide encoding a soluble coenzyme-linked glucose dehydrogenase that catalyzes the oxidation of glucose in the presence of an electron acceptor and has an activity toward maltose of 5% or lower; a polypeptide encoded by the nucleotide sequence of the polynucleotide; a recombinant vector carrying the polynucleotide; a transformed cell produced using the recombinant vector; a method for producing a polypeptide comprising culturing the transformed cell and collecting from the cultivated products a polypeptide that links to FAD to exert the glucose dehydration activity; a method for determination of glucose using the polypeptide; a reagent composition for determination of glucose; and a biosensor. |
申请公布号 |
US2016076007(A1) |
申请公布日期 |
2016.03.17 |
申请号 |
US201514951457 |
申请日期 |
2015.11.24 |
申请人 |
Ikeda Food Research Co., Ltd. |
发明人 |
Omura Hironori;Sanada Hirokazu;Yada Takako;Atsumi Ayaka;Morita Tetsunari;Ishimaru Emi |
分类号 |
C12N9/04 |
主分类号 |
C12N9/04 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
Fukuyama-shi JP |