SYNTHESIS OF PRAZOLE COMPOUNDS

摘要

The present disclosure relates to non-naturally occurring monooxygenase polypeptides useful for preparing prazole compounds, polynucleotides encoding the polypeptides, and methods of using the polypeptides.

主权项

1. A non-naturally occurring polynucleotide sequence encoding a non-naturally occurring monooxygenase comprising a polypeptide capable of converting 5-methoxy-2-(4-methoxy-3,5-dimethylpyridin-2-yl)methylthio)-1H-benzo[d]imidazole (“compound (1)”) to 5-methoxy-2-((4-methoxy-3,5-dimethylpyridin-2-yl)methylsulfinyl)-1H-benzo[d]imidazole (“compound (2)”), and which polypeptide amino acid sequence:
(a) is at least 80% identical to SEQ ID NO: 124; and (b) has two or more residue differences as compared to SEQ ID NO:2 at the following residue positions: position corresponding to X246; position corresponding to position corresponding to X248; position corresponding to X326; position corresponding to X386; position corresponding to X432; position corresponding to X433; position corresponding to X435; position corresponding to X438; and position corresponding to X448.