DNA sequencing using multiple metal layer structure with different organic coatings forming different transient bondings to DNA

摘要

A nanodevice includes a reservoir filled with conductive fluid and a membrane separating the reservoir. A nanopore is formed through the membrane having electrode layers separated by insulating layers. A certain electrode layer has a first type of organic coating and a pair of electrode layers has a second type. The first type of organic coating forms a motion control transient bond to a molecule in the nanopore for motion control, and the second type forms first and second transient bonds to different bonding sites of a base of the molecule. When a voltage is applied to the pair of electrode layers a tunneling current is generated by the base in the nanopore, and the tunneling current travels via the first and second transient bonds formed to be measured as a current signature for distinguishing the base. The motion control transient bond is stronger than first and second transient bonds.

主权项

1. A system, comprising:
a nanodevice, comprising:
a reservoir filled with a conductive fluid;a membrane separating the reservoir, the membrane comprising a plurality of electrode layers including a first pair of electrode layers and a second electrode layer, wherein each of the plurality of electrode layers is separated by an insulating layer; anda nanopore formed through the membrane, wherein the first pair of electrode layers is coated inside the nanopore with a first type of organic coating and the second electrode layer is coated inside the nanopore with a second type of organic coating to form a motion control transient bond, wherein the first type of organic coating comprises a functionality of resorcinol and the second type of organic coating comprises a functionality of at least one of phosphonic acid and hydroxamic acid; a voltage source configured to apply a voltage;
wherein, in response to application of the voltage by the voltage source to the first pair of electrode layers, a tunneling current is generated by a base of nucleic acid in the nanopore;wherein the tunneling current traveling via a first transient bond and a second transient bond formed between the first pair of electrodes and the base of the nucleic acid is measured as a current signature for distinguishing the base; andwherein the motion control transient bond is stronger than the first and second transient bonds.