Modified cell lines for increasing lentiviral titers

摘要

The present disclosure provides a method of modifying cells in order to enhance lentiviral titers, cell lines that are modified and modifying reagents. By mediating individual genes and combination thereof, lentiviral titers may be increased.

主权项

1. A method of enhancing lentiviral titers comprising modulating expression of a plurality of host genes in a packaging cell, wherein the modulation comprises”
a) suppressing expression of Tetherin, and b) at least one of
i) suppressing expression of at least one of Dicer and DGCR8, andii) increasing expression of at least one of TSG101 and Anx2.