PROTEIN DERIVATIZATION TO ENDOW CELL PENETRATION

摘要

Methods and reagents for enhancing cellular uptake of a cargo molecule by covalently bonding optionally-substituted fluorenyl groups to the cargo molecules, where cellular uptake includes at least partial uptake into the cytosol. Useful fluorenylation reagents include those of formula:;;and salts thereof where variables are as defined. Cargo molecules include peptides and proteins. Also provided are fluorenylated cargo molecules, including fluorenylated peptides and proteins.

主权项

1. A method for increasing cellular uptake of a cargo molecule by fluorenylating the cargo molecule by covalently bonding one or more optionally substituted fluorenyl groups to the cargo molecule and contacting a cell with the fluorenylated cargo molecule.