| 发明名称 |
GENOME EDITING IN RATS USING ZINC-FINGER NUCLEASES |
| 摘要 |
Disclosed herein are methods and compositions for genome editing of one or more loci in a rat, using fusion proteins comprising a zinc-finger protein and a cleavage domain or cleavage half-domain. Polynucleotides encoding said fusion proteins are also provided, as are cells comprising said polynucleotides and fusion proteins. |
| 申请公布号 |
US2016068865(A1) |
申请公布日期 |
2016.03.10 |
| 申请号 |
US201514946224 |
申请日期 |
2015.11.19 |
| 申请人 |
Sigma-Aldrich Co. LLC ;Sangamo BioSciences, Inc. |
发明人 |
Cui Xiaoxia;Geurts Aron M.;Urnov Fyodor |
| 分类号 |
C12N15/90;C12N15/85;C12N15/10 |
主分类号 |
C12N15/90 |
| 代理机构 |
|
代理人 |
|
| 主权项 |
1. A method for modifying one or more endogenous cellular genes in a rat cell, the method comprising: introducing, into the rat cell, one or more polynucleotides encoding one or more zinc finger nucleases (ZFNs) that bind to a target site in the one or more genes under conditions such that the ZFN(s) is (are) expressed and the one or more endogenous cellular genes are cleaved and modified. |
| 地址 |
St. Louis MO US |
