Compositions and methods for preparation of synthetic alpha-gal nanoparticles and for their clinical use

摘要

The present invention is related to the field of healing of internal injuries. In particular, the present invention provides compositions and methods comprising molecules and nanoparticles with linked α-gal epitopes from synthetic origin for induction of recruitment and activation of macrophages within or surrounding injured tissue of treated patients. These macrophages further recruit stem cells into the injured tissues. The recruited macrophages and stem cells promote the repair and regeneration of the treated injured tissue. This invention further teaches methods and compositions comprising molecules and nanoparticles with linked α-gal epitopes of synthetic origin for inducing recruitment and activation of macrophages into biomaterial implants for improving the conversion of such implants into functional tissues and organs within treated patients.

主权项

1. A method for inducing recruitment of macrophages into an injured internal or external tissue and for activation of said recruited macrophages to produce pro-healing cytokines and growth factors in a subject having endogenous anti-Gal antibody, comprising: administering to said injured internal or external tissue a composition that comprises synthetic α-gal nanoparticles, wherein:
i) said synthetic α-gal nanoparticles comprises α-gal epitope having a terminal α-galactosyl, and ii) said composition is administered under conditions that increases an amount of macrophages in said injured internal or external tissue of said subject.