STRAND EXCHANGE HAIRPIN PRIMERS THAT GIVE HIGH ALLELIC DISCRIMINATION

摘要

Provided herein are compositions and methods for identification of the presence or absence of a particular sequence, such as a single nucleotide polymorphism. Employed herein are particular primers that comprise a hairpin and a single strand extension at the 3' end, the single strand extension in which at least one nucleotide is mismatched compared to a target particular sequence. Strand displacement that leads to additional binding of the primer and extension of the primer occurs following initial binding of the primer to the nucleic acid comprising the particular sequence.