IMMUNOTHERAPY OF CANCER THROUGH COMBINATION OF LOCAL AND SYSTEMIC IMMUNE STIMULATION

摘要

Provided herein are compositions and methods for immunotherapy of cancers, said methods combining systemic vaccination with a recombinant expression vector encoding a viral antigen and/or a cancer specific tumor antigen(s) to induce activated CD8 T-cells, with a local (e.g., intratumoral) immune stimulation using standard or modified application of a TLR agonist, to induce local inflammatory and innate immune responses which recruit T-cells to the tumor.

主权项

1. A method of treating a cancer comprising:
a) administering to a subject a first composition comprising a vector particle, the vector particle comprising a recombinant expression vector wherein the recombinant expression vector comprises a polynucleotide encoding a cancer-associated antigen, and wherein the polynucleotide is operatively linked to at least one regulatory expression sequence, thereby inducing an immune response against the cancer-associated antigen in the subject; and b) administering intratumorally, peritumorally, intranodally or intravesically to the subject a second composition comprising a TLR4 agonist wherein the composition does not comprise antigen; wherein the first composition and the second composition each further comprise a pharmaceutically suitable excipient, and wherein the first composition and second composition are administered concurrently or sequentially.