Use of <i>Galerina marginata </i>genes and proteins for peptide production

摘要

The present invention relates to compositions and methods comprising genes and peptides associated with cyclic peptides and cyclic peptide production in mushrooms. In particular, the present invention relates to using genes and proteins from Galerina species encoding peptides specifically relating to amatoxins in addition to proteins involved with processing cyclic peptide toxins. In a preferred embodiment, the present invention also relates to methods for making small peptides and small cyclic peptides including peptides similar to amanitin. Further, the present inventions relate to providing kits for making small peptides.

主权项

1. A fungus cell transfected with a recombinant prepropeptide nucleic acid encoding a proline-containing peptide operably linked to a promoter, wherein the fungus cell comprises a fungal prolyl oligopeptidase nucleic acid encoding an amino acid sequence selected from the group consisting of SEQ ID NO: 236, 237, 348, 716, and 722.